MIP based Electrochemical Identification of Rutin using Lead Pencil as An Electrode Source.

Abstract

In response to the increasing demand for rapid and user-friendly procedures in research laboratories and hospitals, this study introduces a novel and direct electropolymerization method for monitoring Rutin in biological samples and pharmaceutical formulations. A molecularly imprinted pencil graphite electrode was employed, and electropolymerized in situ to create a Molecularly Imprinted Polymer (MIP) network. The electropolymerization process involved Rutin as the template, pyrrole as the monomer, and SnS2/rGO. Experimental parameters were fine-tuned using Differential Pulse Voltammetry (DPV) to optimize MIP efficacy. DPV investigations demonstrated a proportional increase in the peak oxidation signal with decreasing Rutin concentrations, showcasing the sensitivity of the developed method. Rutin concentrations ranging from 0.05nM to 100 µM could be accurately measured, boasting an impressive low detection limit of 0.01 nM. The proposed sensor exhibited exceptional detection capabilities in biological samples spiked with Rutin, highlighting acceptable recovery rates. This innovative electrochemical approach, combining molecular imprinting with electropolymerization, not only offers a sensitive and selective method for Rutin detection but also presents a versatile platform for monitoring other bioactive compounds. The simplicity, efficiency, and low detection limit make it a promising tool for routine analysis in diverse healthcare and pharmaceutical applications.