Development Of Visual Biosensor For The Detection Of Alcohol Contamination In Food And Beverages

Abstract

A biosensor based on Peroxidase (POD) immobilized onto Polyaniline film (PANI) for determination of ethanol contamination in beverages and food is described. The biosensor changes color from green to blue when come in contact with ethanol by producing acetaladehyde and hydrogen peroxide via enzyme catalyzed reaction. The produced hydrogen peroxide oxidizes the PANI film. To obtain this biosensor, the extraction of Peroxidase (POD) from Ipomoea batatas (sweet potatoes) is first described. The activity of crude extract of Peroxidase was determined spectrophotometrically before and after centrifugation. The extracted Peroxidase (POD) was immobilized onto Polyaniline (PANI) film by adsorption method. The solution of Peroxidase (POD) was deposited on PANI film and left for 30 minutes for drying purposes. The biosensor was fabricated in the form of dipping strips for simple, easy and visual use. The color changes of the films were analyzed through image analysis software (ImageJ) to get the amount of ethanol in samples. The mean RGB values resulted in the quantification of ethanol in different samples. The resulted biosensor showed accurate results for ethanol determination. Therefore it can be used for simple and visual to detect ethanol in foods and drinks as well as for Muslim countries for halal verification.