Abstract:
Cancer is one of the leading cause of death worldwide and delay in diagnosis is one of the
major reason of high mortality rate. Among different cancer biomarkers interleukin-8 (IL-
8) is considered as useful biomarker for the purpose of quick and accurate diagnosis. A
sensitive and selective interdigital transducer based sensor have been fabricated by using
molecular imprinted polymer (MIP) technique for the detection of biomarker IL-8.
Detection sites synthesized by using functional monomer Methylmethacrylate (MMA),
Graphene Oxide GO, cross linker Ethylene glycol dimethacrylate (EGDMA) and
IRGACURE blend as a photo initiator. The synthesized sensor showed a high sensitive
response for a linear range of IL-8 concentration 1ng/ml to 500ng/ml and coefficient of
determination of R2 = 0.954 confirm the linear detection behavior with 239 pg/ml limit of
detection. It also showed high selectivity Ribonuclease, IL-6, Lysozyme, Chymotrypsin
and Glycoprotein used as control proteins. This sensor showed a unique strategy for
detection of IL-8 in buffer as well as in saliva. Scanning electron microscopy (SEM) has
been used to analyze the morphology of the fabricated sensor surface.
