Fabrication of the Sensor Device for the Detection of Cancer Biomarker

Abstract

Cancer is one of the leading cause of death worldwide and delay in diagnosis is one of the major reason of high mortality rate. Among different cancer biomarkers interleukin-8 (IL- 8) is considered as useful biomarker for the purpose of quick and accurate diagnosis. A sensitive and selective interdigital transducer based sensor have been fabricated by using molecular imprinted polymer (MIP) technique for the detection of biomarker IL-8. Detection sites synthesized by using functional monomer Methylmethacrylate (MMA), Graphene Oxide GO, cross linker Ethylene glycol dimethacrylate (EGDMA) and IRGACURE blend as a photo initiator. The synthesized sensor showed a high sensitive response for a linear range of IL-8 concentration 1ng/ml to 500ng/ml and coefficient of determination of R2 = 0.954 confirm the linear detection behavior with 239 pg/ml limit of detection. It also showed high selectivity Ribonuclease, IL-6, Lysozyme, Chymotrypsin and Glycoprotein used as control proteins. This sensor showed a unique strategy for detection of IL-8 in buffer as well as in saliva. Scanning electron microscopy (SEM) has been used to analyze the morphology of the fabricated sensor surface.