Investigation of Influence of Thiol Groups on the Drug Loading and Release Properties of Thiolated Derivatives of Cyclodextrin

Abstract

The purpose of this work is to investigate the potential effect of the thiolated and unmodified β-CD on the encapsulation and release efficiency by making its complexes with the drugs. For this investigation, first oxidation of β-CD was done followed by the cysteamine conjugation to β-CD. Ellman’s test was performed to evaluate the no. of thiol groups and disulfide bonds in the thiolated β-CD. Then three drugs namely Fluconazole, Vancomycine and Minoxidil were used to make the inclusion complexes. On one hand their complexes were made with the non-thiolated-β-CD and on the other hand, their complexes were made with thiolated-β-CD. FTIR and XRD analysis were done to check the interactions between the complexes. Drug loading capacity was analyzed by UV Spectrophotometer which illustrated that the thiolated-β-CD complexes have increased drug loading capacity. Drug release time get significant effect in a way that the sustained release of drugs was found for the thiolated-β-CD complexes.