Exploring the Potential of Mn/Zno@Mxene Nanocomposite in Biomedical Applications

Abstract

Mxenes are two-dimensional transition metal composed of carbides and nitrides, which have recently attracted much attention as biomedical application platforms owing to their high electrical conductivity, numerous surface functional groups, and biocompatibility, which is favorable, giving them the best places to design multifunctional nanocomposites. This paper has managed to prepare and characterize a MnZno@Mxene nanocomposite through XRD, FTIR, Raman spectroscopy, UV-Vis spectroscopy, SEM, EDX, elemental mapping, and XPS. The MnZno@Mxene nanocomposite was found to have great antibacterial activity against both Gram-positive and Gram-negative bacterial with the maximum inhibitory zone of 22 mm against Escherichia coli, 18 mm against Staphylococcus aureus, and 19 mm against Pseudomonas aeruginosa, as compared to the standard antibiotics, including gentamicin and ciprofloxacin, and time-kill kinetic analysis revealed successful and sustained bacterial inhibition, especially in E. coli. A wound-healing assessment of a full-thickness excisional wound model in vivo showed that the treated group had a significantly faster healing rat with 95.19% wound closure at 10 days as opposed to 66.76% in the negative control, as well as increased skin regeneration and improved wound morphology. In addition, the nanocomposite also showed significant anti-inflammatory effects in carrageenan induced paw edema model, with paw thickness reducing from 4.39 mm to 6.96 mm after 24 hours, as a result, a change similar to the positive control. The high potential of MnZno@Mxene was further validated by histopathological analysis, which revealed increased re- epithelialization, decreased inflammation, and ordered deposition of collagen in treated tissues, indicating high adaptability and usefulness of MnZno@Mxene as an antimicrobial therapy, wound healing, and inflammation control material.