Anti-Biofilm Activity of Medicinal Plants Extracts against Burn Isolates

Abstract

Out of complications, burnt skin is a leading medical, social and economic problem. Proper management of burn wounds is complicated and involves a lot of necessary measures. This study aims at evaluation of clinical and microbial characteristics of burn infections and effects of Ziziphus mauritiana extracts in curing and healing process of burns. Burn wound healing is complicated phenomenon which includes the processes for tissue repairing, elimination of inflammation, granulation, epithelialization and wound contraction. The infections caused by the certain bacteria have become a major challenge in the treatment of burn wounds and has become associated with the morbidity and mortality rates in hospitals. Despite the advances in the medication processes, a great number of world populations still depend upon the traditional medicines. The traditional herbal methods used for the treatment of burns wounds have antioxidant, anti-inflammatory and angiogenic effects. The most common individual isolates in burn patients are Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus mirabilis, Citrobacter sp. Escherichia coli and Staphylococcus aureus. Out of these, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli were selected for the following study. These organisms were sensitive as well as resistant to most of antibiotics. The biofilms of Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli were made on glass substrata from the samples taken from the burn wounds by using qualitative and quantitative methods. Activity of Ziziphus mauritiana leaves extracts against the organisms in the form of biofilm was estimated. This study aims at evaluation of Anti-biofilm activity of Ziziphus mauritiana extracts could be developed as an organic medication and safe treatment method for the burn patients.