Synthesis and Characterization of NiFe2O4/CS Nanocomposites and its application towards Choline Biosensing

Abstract

A non-enzymatic choline biosensor was demonstrated with chitosan (CS) supported NiFe2O4 modified on carbon paste electrode (CPE). The NiFe2O4 was synthesized via hydrothermal method to study the choline chloride (ChCl) behaviour towards the distinctive performance of choline. The prepared sample presented cost-effective choline sensing as emerged with CS formed NiFe2O4/CS and immersed with CPE. The synthesized NiFe2O4 was examined with different characterization technique to confirm the formation of specified material. X-ray diffraction (XRD), Fourier- Transmission Infrared spectroscopy (FTIR) and Raman Spectroscopy used to optimize and evaluate the various properties of prepared material. XRD confirmed the cubic inverse spinel crystal structure of NiFe2O4 with crystallite size of 14nm, while FTIR spectra revealed the stretching and bending vibration over the IR frequency range and modes confirmed through Raman spectra frequency range. The modified electrode of NiFe2O4/CS/CPE was used to perform electrochemical studies by voltammogram and evaluated through Cyclic voltammetry (CV). The CV curves taken at different working range (5-15μL) of potential 0.0-+1.0 V and quantified the analyte signal of LoD (0.002μM) considering the S/N=3 across the current system with the linear regression (R2) of 0.99. The prepared electrode showed the low (LoD) at the smaller range of the choline chloride and demonstrated good response