METAL NANOPARTICLES DECORATED 2D NANO-MATERIALS FOR BIOSENSING APPLICATIONS

Abstract

This thesis aims to develop a novel fluorescence-based sensor for UA detection by integrating MX/CoNFs composites with Rhodamine dye. The sensor is designed to exhibit high sensitivity, selectivity, and stability, overcoming limitations of traditional enzymatic assays. This work begins with the synthesis of MXene nanosheets and CoNFs, followed by the fabrication of the nanocomposite. Comprehensive characterization techniques such as UV-Vis spectroscopy, SEM, XRD, and fluorescence spectroscopy were employed to confirm the structural and optical properties of the materials. The fluorescence sensing mechanism is based on the quenching of Rhodamine fluorescence by the MX/CoNFs nanocomposite, and its subsequent recovery upon the introduction of UA. This “turn-off” response is utilized to quantify UA levels. Various parameters affecting sensor performance, including dye concentration, quenching efficiency, sensitivity, and selectivity against interfering substances, were systematically optimized. The developed sensor is promising a good candidate for practical applications in clinical diagnostics. This work highlights the potential of MXene-based nanohybrids in biosensing and contributes to the growing field of fluorescence spectroscopy for biochemical detection.