Fabrication & Characterization of Ti-Ce MOFs based silk electro spun membrane for bone Regeneration A combined DFT and Experimental Approach

Abstract

Neurosurgical procedures, traumatic brain injury, tumor resection, and cerebrovascular disorders may lead to leaking cerebrospinal fluid and development of severe complications such as infection, inflammation, and dysfunction of the nervous system. The traditional methods of dural repairs like sutures, sealants, and hydrogels are usually constrained by factors such as partial repair, immunogenic response, lack of accessibility and mechanical efficacy. In solving these issues, a mechanically stable, biocompatible and biodegradable scaffold should be developed. In this work we have fabricated a bilayer electrospun scaffold that was created using silk fibroin, calcium magnesium silicates (SILK+CMS), poly(epsilon-caprolactone) (PCL), with metal-organic frameworks (PCL+MOF) to regenerate the dura mater. The scaffolds were prepared and characterized in a systematic way by application of different techniques of analysis. The FTIR analysis showed that all components had characteristic functional groups, which correspond to the successful fabrication of the scaffolds. The bilayer scaffolds also had optimized hydrophilicity, swelling characteristics, porosity, and density that were beneficial in cell attachment, growth, diffusion of nutrients, and tissue integration. SEM examination demonstrated a consistent fibrous pattern and well organization of the fibers which were very similar to the normal dura mater structure. In vitro degradation tests proved that the scaffolds had a programmed rate of degradation that could be adjusted to the rate of healing in the dura mater. Moreover, in vitro biocompatibility tests revealed that there is a good cellular response, which indicates that the scaffold can facilitate cell attachment and viability. Altogether, the findings indicate that the developed bilayer scaffold can be regarded as the promising biomaterial candidate to repair dura mater, and it may help avoid the cerebrospinal fluid leakage and promote successful tissue regeneration