Design and Development of Graphene Oxide Loaded Chitosan Scaffolds for Skin Tissue Regeneration

Abstract

Carbon-based nanomaterials (graphene oxide) are well known for their biomedical applications especially skin tissue regeneration, due to their potential to where they support angiogenesis, their good solution uptake ability, and antibacterial activities. The development of cost-effective chitosan-based materials that can support angiogenesis to tissue healing is highly desired to prepare advanced scaffolds using nanomaterials. The purpose of this study was to evaluate the potential of prepared scaffolds to support angiogenesis towards tissue regeneration. Different composites of chitosan and graphene oxide were prepared by the freeze gelation method. During this process different low concentrations of graphene oxide in-situ in the chitosan solution stirred to get the homogenous solution. The synthesized composite scaffolds were then characterized for their structural, chemical and mechanicals analysis using FTIR and Tensile technique. FTIR analysis confirmed the presence of chitosan functional groups in every scaffolds. Universal Fatigue testing results shows the tensile strength (MPa) and elongation at break (%) was increasing while increasing the concentration to the chitosan. All these experiments confirm that these membranes are suitable to use for tissue regeneration and wound healing applications.