Fabrication of a Sensor Device for Lipoprotein – a Biomarker for Coronary Heart Disease.

Abstract

Very low-density lipoprotein (VLDL) is a biomarker for the coronary heart disease. It is the major cause to build up the arteriosclerotic plaques in the coronary artery. This target material cannot be directly assessed in the clinical laboratory testing because concentration of VLDL depends upon the sensing triglycerides attached in its particles. Molecular imprinting technique was utilized to fabricate a sensor for the direct detection of the VLDL concentration in the human serum. The novel VLDL-MIP was fabricated using β-cyclodextrin and methacrylic acid in the ratio of 1:3 (w/w) as functional monomers in the existence of N, N-Dimethylformamide (DMF) as solvent, ethylene glycol di methacrylate (EGDMA) as a cross linking agent and IRGACURE blend as a photo initiator. The VLDL- MIP sensor showed highly sensitive with the linear response from 1 ng/ml to 500 ng/ml of very low-density lipoprotein concentration. This sensor showed the very low cross- selectivity with the other proteins like glycoprotein, IL8, ribonuclease etc. This sensor is reproducible and repeatable with the coefficient of determination of R2 = 0.9478 with the limit of detection of 3.42 pg/ml. This novel senser showed a unique and new strategy for the detection of VLDL in the serum.