Synthesis of Nickel Oxide (NiO) Fluorescence Nano Quencher and Their Application in Aptasensing of Ochratoxin-A

Abstract

The fabrication of fluorescence based biosensor for the ultra-sensitive and selective monitoring of Ochratoxin-A in the food samples is very important these days. We demonstrate for the first time, the development of nickel oxide nanoparticles (NiO) quenching based aptasensing platform for the detection of target molecule (OTA). In this work, NiO quench the fluorescence of FAM-labeled aptamer. These quenched intensities were due to energy transfer processes to the metal nanoparticles. Conversely, increase in fluorescence quenching intensities with a decrease in the size of the nanoparticles which may be useful for the design of new analytical tool to detect the presence of target analyte. The structural properties of prepared nano materials were analyzed by X- Ray diffraction (XRD).Surface morphology of prepared metal oxide nano materials were studied by Scanning Electron Microscopy (SEM) and crystalline behaviors size/shape was analyzed by RAMAN spectroscopy. We have found the cost-effective, easy to use and affordable method to design aptamer based fluorescence quenching assay for the detection and early diagnoses of mycotoxins (OTA).