Synthesis of Biomolecule Loaded Bioactive Glass and its Antibacterial Properties

Abstract

Bioactive glasses (BGs) are synthetic bone fillers that have ability to replace damaged bone, facilitate tissue regeneration and integrate with body environment. In this study we used bioactive glass prepared by co-precipitation method and used doxycycline as a biomolecule. Doxycycline (DOX) was loaded on bioactive glass with different concentrations (2mg/mL, 4mg/mL and 8mg/mL). Both, simple BG and BG/DOX was characterized using Fourier Transform Infrared spectrophotometer (FTIR), X-Ray Diffraction (XRD), and Scanning Electron Microscopy (SEM). Antibacterial studies were conducted against E. Coli and S. Aureus bacteria. The results showed that BG/DOX was affective against these bacterial strains as they inhibited their growth. The zone of inhibition was 19.5mm, 26mm, 26.5mm and 22.5mm, 28mm, 24mm for BG/DOX-2, BG/DOX-4 and BG-DOX-8 against E. coli and S. aureus respectively. There was not much difference in zone of inhibition by changing concentration from 4mg to 8mg. Hence, 4mg is considered as an optimum concentration. Therefore, Doxycycline drug loaded BGs can be used to enhance the antibacterial properties of BGs, in tissue engineering applications, and in root implants of teeth's for long term effect.