A Porphyrin based Estimation of Histamine Towards Detection of Meat Spoilage

Abstract

A major barrier to clinical diabetes diagnosis, management, and therapy is accurate histamine monitoring in chicken flesh. Due to their exceptional optical, electrical, and detecting qualities, histamine sensors based on fluorescent 2D material nanocomposites have drawn a lot of interest. The zinc oxide nanoparticles (ZnO NPs) developed in the current research display exceptional sensitivity and selectivity with 5,10,15,20-Tetrakis(4-hydroxyphenyl)-21H,23H-porphine (THPP) dye because of their high surface area, increased number of active sites, and improved common optical and electrical features, Using several characterization instruments such as SEM, XRD, FTIR, RAMAN spectroscopy, UV-Vis and Fluorescence Spectrophotometry morphology, and the surface chemistry chemical content, and fluorescence behaviour of the produced samples were justified. In the designed sensor, the fluorescence quenching of the THPP dye with ZnO-NPs when treated with various concentrations of histamine and without histamine, successfully increased the response of the fluorescent dye. The sensor has a linear range of 1.2 mM to 6.7 mM of histamine and a sensitivity of 3.34% mM-1 with the lower limit of detection i.e., 0.089 mM. Through the use of various molar concentrations of histamine and other analytes, the sensor's selectivity and sensitivity have been verified. Accordingly, based on our findings, the suggested sensor has a high level of discernment and responsiveness in its behaviour of fluorescence quenching efficiency towards histamine