Department of Chemistry

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Subject: search.filters.subject.Biomedical Applications

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    Development of a Modified Porphyrin Based Composite for Biomedical Application
    (Library Information Services, COMSATS University Islamabad, Lahore Campus, 2025) Husnain Ali Awan; CIIT/SP24-R06-022/LHR; Dr. M. Shahid Nazir; LHR TP 10026
    Zn-TCPP@CNC, a novel fluorescent nanocomposite was prepared by conjugation of Zn-TCPP and CNC. That Zn2+ ions have been successfully coordinated with the porphyrin core, the key functional groups are retained, and the MOF interacts well with the CNC support have been confirmed by structural and optical characterizations, such as UV-Vis, FTIR, and XRD. FESEM analysis showed rod shaped morphology with MOF particles decorated on the surface, this leads to high surface area and less aggregation of the MOF particles, which is ideal in sensing application. Fluorescence of Zn-TCPP@CNC was studied in the process of selectively detecting ascorbic acid. The material had maximum absorption at 420 nm, and showed fluorescence emission spectra with peaks between 600-700nm, which was effectively quenched with the help of ascorbic acid through a photoinduced electron transfer process. Optimization experiments have shown that a material concentration of 200 mL gave both stable and reproducible fluorescence values. The specificity of the sensor was proved by selectivity experiments, which showed low interference with glucose, fructose, urea, uric acid and biogenic amines. Sensitivity analyses demonstrated that the quenching effect was concentration-dependent, and, therefore, allowed the quantitative determination of ascorbic acid. The slope of the calibration curve and the standard deviation of the blank values were used to determine the limit of detection (LOD) which is 18.29 µM. This is explained by the fact that quenching is because of effective molecular interactions, such as hydrogen bonding and photoinduced electron transfer. These findings suggest that Zn-TCPP@CNC is a sensitive, selective, and reliable platform of fluorescence detection of ascorbic acid.
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    Exploring the Potential of Mn/Zno@Mxene Nanocomposite in Biomedical Applications
    (Library Information Services, COMSATS University Islamabad, Lahore Campus, 2025) Hina Murtaza; CIIT/SP24-R06-007/LHR; Dr. Sadaf Ul Hassan; LHR TP 10016
    Mxenes are two-dimensional transition metal composed of carbides and nitrides, which have recently attracted much attention as biomedical application platforms owing to their high electrical conductivity, numerous surface functional groups, and biocompatibility, which is favorable, giving them the best places to design multifunctional nanocomposites. This paper has managed to prepare and characterize a MnZno@Mxene nanocomposite through XRD, FTIR, Raman spectroscopy, UV-Vis spectroscopy, SEM, EDX, elemental mapping, and XPS. The MnZno@Mxene nanocomposite was found to have great antibacterial activity against both Gram-positive and Gram-negative bacterial with the maximum inhibitory zone of 22 mm against Escherichia coli, 18 mm against Staphylococcus aureus, and 19 mm against Pseudomonas aeruginosa, as compared to the standard antibiotics, including gentamicin and ciprofloxacin, and time-kill kinetic analysis revealed successful and sustained bacterial inhibition, especially in E. coli. A wound-healing assessment of a full-thickness excisional wound model in vivo showed that the treated group had a significantly faster healing rat with 95.19% wound closure at 10 days as opposed to 66.76% in the negative control, as well as increased skin regeneration and improved wound morphology. In addition, the nanocomposite also showed significant anti-inflammatory effects in carrageenan induced paw edema model, with paw thickness reducing from 4.39 mm to 6.96 mm after 24 hours, as a result, a change similar to the positive control. The high potential of MnZno@Mxene was further validated by histopathological analysis, which revealed increased re- epithelialization, decreased inflammation, and ordered deposition of collagen in treated tissues, indicating high adaptability and usefulness of MnZno@Mxene as an antimicrobial therapy, wound healing, and inflammation control material.