Department of Chemistry
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Item Chitosan-PCL-Bioactive Glass-Vitamin E based Scaffold for bilayer periosteum regeneration(Library Information Services COMSATS University Islamabad Lahore Campus, 2021-02-24) RAKHSHANDA ASHRAF; FA19-R06-019; Dr. Hamad Khalid; LHR TP 7330The periosteum is a dense, well-vascularized membrane that covers the exterior surfaces of bones, except for the joints. The periosteum is responsible for collagen arrangement and cell regulation, and it is important for bone integrity, modelling, remodeling particularly in bone healing. Periosteum transplantation has been used in clinical medicine due to its superior ability to deliver osteoprogenitor cells while also protecting scar tissues. Immunological rejection, periosteum availability, and donor side morbidity are, as with previous transplants, the key issues. It is envisaged that the use of tissue engineering ideas in periosteum-related therapy would increase the use of periosteum in bone therapies. It can also act as a substitute for natural periosteum. In this study, we have prepared scaffold for bilayer periosteum incorporated with Chitosan(CS), Polycaprolactone(PCL), Bioactive glass (BG) and Vitamin E (Vit E) through Freeze drying and Electrospinning methods. FTIR was used for detection of functional groups of scaffold. The physiological behavior was also investigated such as degradation, swelling behavior and porosity. With a suitable degradation rate, the porosity of composite scaffolds was determined to be in the range 100 percent. Invitro cell studies indicated that scaffold support growth and cell adhesion. The innovative composite scaffolds will be promising candidates for bilayer periosteum regeneration.Item Fabrication and Characterization of Tri-Layered Electrospun Membranes for Bone Regeneration(Library Information Services, COMSATS University Islamabad, Lahore Campus., 2025-04-01) Hafiza Sunaina Ijaz; CIIT/FA23-R06-011/LHR; Dr. Hamad Khalid; LHR TP 9687This study centres on the development and detailed evaluation of a tri-layered electrospun membrane designed to mimic natural tissue architecture for bone regeneration applications. The membranes consist of three unique layers: the outer layer is made of PLA embedded with Zr-ZnO nanoparticles, the middle core layer comprises PCL infused with Vitamin E, and the innermost layer is formed from Silk Fibroin (SF) incorporated with Si-HA to promote antibacterial activity, angiogenesis, and osteogenesis, respectively. To comprehensively assess the structural and functional properties of the scaffold, a range of characterization tools was employed, including FTIR, SEM, contact angle testing, dynamic mechanical analysis, micro-computed tomography, and porosity measurements. These analyses confirmed the presence of uniform fiber formation, interconnected pore networks, surface hydrophilicity, and mechanical flexibility across the tri-layered structure. Biological assessments in vitro involved antibacterial activity, material degradation behaviour, swelling capacity, and cell-based assays using osteoblast-like cells. The membrane demonstrated minimal cytotoxic effects, strong antibacterial performance, and high cell viability. Additionally, cell adhesion and migration were notably enhanced, as observed through staining techniques and migration analysis. Overall, the findings highlight the potential of this multi-layered electrospun scaffold as a viable candidate for bone repair applications. Its design effectively merges mechanical strength, biodegradability, and biological activity, marking a meaningful advancement in the field of regenerative biomaterials