M.Phil / MS
Permanent URI for this collectionhttps://repository.cuilahore.edu.pk/handle/123456789/30
This collection archives the complete set of theses produced by students of the COMSATS University Islamabad, Lahore Campus.
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Item Fabrication of the Sensor Device for the Detection of Cancer Biomarker(Library Information Services COMSATS University Islamabad Lahore Campus, 2021-02-25) Shaista Ahmed; FA19-R06-005; Dr. Usman Latif; LHR TP 7327Cancer is one of the leading cause of death worldwide and delay in diagnosis is one of the major reason of high mortality rate. Among different cancer biomarkers interleukin-8 (IL-8) is considered as useful biomarker for the purpose of quick and accurate diagnosis. A sensitive and selective interdigital transducer based sensor have been fabricated by using molecular imprinted polymer (MIP) technique for the detection of biomarker IL-8. Detection sites synthesized by using functional monomer Methylmethacrylate (MMA), Graphene Oxide GO, cross linker Ethylene glycol dimethacrylate (EGDMA) and IRGACURE blend as a photo initiator. The synthesized sensor showed a high sensitive response for a linear range of IL-8 concentration 1ng/ml to 500ng/ml and coefficient of determination of R2 = 0.954 confirm the linear detection behavior with 239 pg/ml limit of detection. It also showed high selectivity Ribonuclease, IL-6, Lysozyme, Chymotrypsin and Glycoprotein used as control proteins. This sensor showed a unique strategy for detection of IL-8 in buffer as well as in saliva. Scanning electron microscopy (SEM) has been used to analyze the morphology of the fabricated sensor surfaceItem Fabrication of a Sensor Device for Lipoprotein – a Biomarker for Coronary Heart Disease.(Library Information Services COMSATS University Islamabad Lahore Campus, 2021-02-24) Muhammad Talha Talib; FA19-R06-033; Dr. Usman Latif; LHR TP 7336Very low-density lipoprotein (VLDL) is a biomarker for the coronary heart disease. It is the major cause to build up the arteriosclerotic plaques in the coronary artery. This target material cannot be directly assessed in the clinical laboratory testing because concentration of VLDL depends upon the sensing triglycerides attached in its particles. Molecular imprinting technique was utilized to fabricate a sensor for the direct detection of the VLDL concentration in the human serum. The novel VLDL-MIP was fabricated using β-cyclodextrin and methacrylic acid in the ratio of 1:3 (w/w) as functional monomers in the existence of N, N-Dimethylformamide (DMF) as solvent, ethylene glycol di methacrylate (EGDMA) as a cross linking agent and IRGACURE blend as a photo initiator. The VLDL- MIP sensor showed highly sensitive with the linear response from 1 ng/ml to 500 ng/ml of very low-density lipoprotein concentration. This sensor showed the very low cross- selectivity with the other proteins like glycoprotein, IL8, ribonuclease etc. This sensor is reproducible and repeatable with the coefficient of determination of R2 = 0.9478 with the limit of detection of 3.42 pg/ml. This novel senser showed a unique and new strategy for the detection of VLDL in the serum.