Browsing by Author "LHR TP 6494"

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Enzymatic Removal of Biofilm from the Clinical Isolates of Diabetic Foot Ulcers
    (Library Information Services COMSATS University Islamabad Lahore Campus, 2020-02-19) Aroosa Sadaf; FA18-R06-015; Dr. Muhammad Javid Iqbal; LHR TP 6494
    Diabetes Mellitus is an aggregation of metabolic disorder in which increase in blood glucose level is reported for a prolonged period which cause many complications in humans. Diabetic foot ulcer (DFU) is one of the main complications which caused by Diabetes Mellitus. Diabetic foot ulcer has severe medical, economic and social effects for prolonged time. Proper management of burn wounds is complicated and involves a lot of necessary measures. This study aims at evaluation of clinical and microbial characteristics of diabetic foot ulcers and effects of Protease enzyme in curing and healing process of these ulcers. Diabetic foot ulcers healing is complicated phenomenon which involves many complicated processes for tissue repairing. The infections caused by the certain bacteria have become a major challenge in the treatment of foot ulcers and has become associated with the morbidity and mortality rates in hospitals. Despite the advances in the medication processes, there is no reliable and successful treatment method for the diabetic foot ulcers. The most common individual isolates in foot ulcers are Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Citrobacter sp. and Escherichia coli. Out of these strains Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were selected for this study. These organisms were sensitive as well as were resistant to most of antibiotics. The biofilms of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were made on glass substrata from the samples taken from foot ulcers by using qualitative and quantitative methods. Activity of crude protease enzyme against the isolates in the form of biofilm was estimated. This study aims at evaluation of Anti-biofilm activity of protease enzyme that could be developed as an alternative, fast and safe treatment method for the diabetic foot ulcers.