Browsing by Author "Dr. Usman Latif"

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Designing of a Sensor for Monitoring Drug in Spiked Serum Samples – Preventing Drug Toxicity
(2025) Amina Sarwar CIIT/FA23-R06-004/LHR; Dr. Usman Latif; LHR TP 9682
This research introduces a MIP based electrochemical sensor for theophylline detection, utilizing a pencil graphite electrode (PGE) modified with ZnO Nano Flakes, reduced graphene oxide (rGO), and pyrrole which undergoes in-Situ chemical reaction to form polymerized layer. Molecular imprinting provided selective binding sites for theophylline, enabling high specificity. The sensor achieved a low detection limit of 0.94nM, with strong selectivity against similar compounds and excellent reusability as MIP based can be used repeatedly. Its fast response and simple fabrication make it ideal for real-time, point-of-care drug monitoring
Detection of Neurotransmitter by Using Molecular Imprinting Technique
(Library Information Services COMSATS University Islamabad Lahore Campus, 2023-02-27) Maleeha Muhammad Din; SP22-R06-014; Dr. Usman Latif; LHR TP 8653
A unique molecularly imprinted polymer (MIP) film was built on a pencil graphite electrode (PGE), by electropolymerization of pyrrole (monomer) consisting of acetylcholine (template) and ZnO.CuO nanoleaves (NLs). The sensor was modified for electrochemical non-enzymatic detection of acetylcholine. Acetylcholine (ACh) serves as an important neurotransmitter and deviation from its normal level contributes to several neurological diseases. The MIP’s experimental parameters were optimized, and the imprinting effect was verified by contrasting the electrochemical reaction of molecularly imprinted polymer (MIP), with that of non-imprinted polymer (NIP). Among the electrochemical sensors the MIP (PPy. ZnO.CuO NLs)/PGE electrochemical sensor manifested the best performance. It showed a wide linear range from 100.0 picomolar (pM) to 100.0 millimolar (mM). The limit of detection (LOD) and limit of quantification (LOQ) were shown to be 9.4 pM and 28.68 mM, correspondingly.
Fabrication of a Sensor Device for Lipoprotein – a Biomarker for Coronary Heart Disease.
(Library Information Services COMSATS University Islamabad Lahore Campus, 2021-02-24) Muhammad Talha Talib; FA19-R06-033; Dr. Usman Latif; LHR TP 7336
Very low-density lipoprotein (VLDL) is a biomarker for the coronary heart disease. It is the major cause to build up the arteriosclerotic plaques in the coronary artery. This target material cannot be directly assessed in the clinical laboratory testing because concentration of VLDL depends upon the sensing triglycerides attached in its particles. Molecular imprinting technique was utilized to fabricate a sensor for the direct detection of the VLDL concentration in the human serum. The novel VLDL-MIP was fabricated using β-cyclodextrin and methacrylic acid in the ratio of 1:3 (w/w) as functional monomers in the existence of N, N-Dimethylformamide (DMF) as solvent, ethylene glycol di methacrylate (EGDMA) as a cross linking agent and IRGACURE blend as a photo initiator. The VLDL- MIP sensor showed highly sensitive with the linear response from 1 ng/ml to 500 ng/ml of very low-density lipoprotein concentration. This sensor showed the very low cross- selectivity with the other proteins like glycoprotein, IL8, ribonuclease etc. This sensor is reproducible and repeatable with the coefficient of determination of R2 = 0.9478 with the limit of detection of 3.42 pg/ml. This novel senser showed a unique and new strategy for the detection of VLDL in the serum.
Fabrication of Nanostructured Receptors-based Device for the Detection of Dengue Fever
(Library Information Services COMSATS University Islamabad Lahore Campus, 2020-02-17) ANUM TAHIR; SP19-R06-030; Dr. Usman Latif; LHR TP 6525
Dengue belongs to a family of Flaviviridae virus and is a single-stranded, positive, enveloped RNA virus having four serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. DENV infection generates ten viral protein products that have nonstructural components (NS1, NS5, NS2A, NS4A, NS2B, NS4B and NS3) and structural components (envelope, capsid and pre-membrane). NS1 is an efficiently used biological marker for the diagnostic purpose of the early stages of dengue. This is because of its presence in all the serotypes of the dengue and its secretion before the production of antibodies against the virus. NS1 dengue viral protein is secreted and accumulated in extracellular space as a hexametric lipoprotein specie. Development of various methods has been reported against dengue’s nonstructural protein 1(NS1) such as detection of viral RNA through a reverse transcription-polymerase chain reaction, serological test, viral culture, enzyme-linked immunosorbent assay, etc. However, these conventional techniques lead to unacceptable time delays, cost, sensitivity, selectivity, and energy. To eliminate these pitfalls, researchers are intending to develop a proficient biosensor for diagnosing NS1 that possess high specificity, sensitivity, and simplicity. Therefore, it is imperative to develop a simple, sensitive, and selective diagnostic technique for the detection of dengue. Molecular imprinting sensors have been developed due to their good sensitivity and specific molecular recognition ability. This facile, robust, and nonbiological approach has been used to detect large molecules such as protein. The main drawback of using proteins as templates is their complex and flexible three-dimensional structure which unfolds easily with minimal changes in the environment. In addition, it is difficult to elute template molecules embedded in molecularly imprinted polymers. Therefore, stable and rigid 3D proteins are strongly preferred for imprinting purposes. The most promising strategy to overcome the complications is to decrease the complexity of the template to achieve successful protein imprinting. Hence, an elegant approach for a new, inexpensive protein imprinting method has been reported in recent years under the x name of epitope imprinting. This imprinting technique involves the use of a small fragment of target protein (epitope) as a template resulting in the formation of MIP layer which will be able to identify the whole protein molecule.
Fabrication of the Sensor Device for the Detection of Cancer Biomarker
(Library Information Services COMSATS University Islamabad Lahore Campus, 2021-02-25) Shaista Ahmed; FA19-R06-005; Dr. Usman Latif; LHR TP 7327
Cancer is one of the leading cause of death worldwide and delay in diagnosis is one of the major reason of high mortality rate. Among different cancer biomarkers interleukin-8 (IL-8) is considered as useful biomarker for the purpose of quick and accurate diagnosis. A sensitive and selective interdigital transducer based sensor have been fabricated by using molecular imprinted polymer (MIP) technique for the detection of biomarker IL-8. Detection sites synthesized by using functional monomer Methylmethacrylate (MMA), Graphene Oxide GO, cross linker Ethylene glycol dimethacrylate (EGDMA) and IRGACURE blend as a photo initiator. The synthesized sensor showed a high sensitive response for a linear range of IL-8 concentration 1ng/ml to 500ng/ml and coefficient of determination of R2 = 0.954 confirm the linear detection behavior with 239 pg/ml limit of detection. It also showed high selectivity Ribonuclease, IL-6, Lysozyme, Chymotrypsin and Glycoprotein used as control proteins. This sensor showed a unique strategy for detection of IL-8 in buffer as well as in saliva. Scanning electron microscopy (SEM) has been used to analyze the morphology of the fabricated sensor surface