Browsing by Author "Dr. Muhammad Hammad Aziz"

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A Non – Enzymatic Hydrogen Peroxide Biosensor Based on RGO/MnO2 Nanocomposite
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2020) Fahad Abbas; P19-RPH-002; Dr. Muhammad Hammad Aziz; LHR TP 6366
Another electrocatalyst, RGO/MnO2 half breed nano structure was effectively incorporated for the non-enzymatic discovery of H2O2. The morphological portrayal was inspected by X-Ray Diffractometery (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The RGO/MnO2 based terminals demonstrated high electrochemical action in order to recognize the hydrogen peroxide in soluble solution. The non-enzymatic biosensors showed great execution alongside small operational potential, great affectability, little recognition boundary, and long haul dependability, that can be ascribed to the great surface area of RGO accommodating the statement of MnO2 nanoparticles. These outcomes show that , fresh Nano composite along great surface territory and electrocatalytic action offers extraordinary guarantee for new class of nanostructured terminal for non-enzymatic biosensor also, energy change applications. Here, in this experimental study, we have synthesized and characterized RGO-MnO2 by X-ray Diffractometery (XRD) , Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM) for structural and morphological studies.
Development of Chitosan Hydrogel Membrane Incorporated Au Doped SnO2 Nanoparticles for Antibacterial Activity and Wound Healing Application
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2021) Momina Rehman; CIIT/SP20-RPH-010/LHR; Dr. Muhammad Hammad Aziz; LHR TP 7709
Metallic nanoparticles are well known for their biomedical applications including antimicrobial activity and wound dressing materials due to large surface to volume ratio and other chemical properties. In this work, gold doped tin oxide (Au-SnO2) nanoparticles containing chitosan-based porous membranes were prepared by the freeze-gelation method. Hydrogel membranes were prepared using the blending of natural polymers and loading metallic nanoparticles as an antibacterial agent. The different membranes were prepared using the different concentrations of metallic nanoparticles by the freeze-gelation process, during this process Triethyl Orthoformate (TEOF) used as a cross-linker agent. This different concentration of membranes were tested for bactericidal potential using the disk diffusion method. Further, swelling studies of these membranes were performed in PBS solutions. The presence of Au-SnO2 NPs were confirmed by the scanning electron microscopy (SEM) analysis. The Fourier Transform Infrared Spectroscopy (FTIR) analysis confirmed the presence of the chitosan functional group. All these experiments confirmed that these membranes are safe to use for wound dressing material and other biomedical applications.
GO/MnFe2O4mediated Electrochemical Biosensing for Monitoring Glucose
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2020) Muhammad Ashfaq; CIIT/SP19-RPH-039/LHR; Dr. Muhammad Hammad Aziz; LHR TP 6376
GO-MnFe2O4 nanoparticles have been used as a drug delivery in medical, biosensing and bioimaging. In this study, GO-MnFe2O4 nanoparticles were prepared by Co precipitation method with variation in stirring time. The prepared material was characterized by XRD, SEM and FTIR. Single phase composite material examined by XRD and average crystallite size found to be 60nm.SEM images shows the proposed material is homogeneous and dense. The working electrode GO-MnFe2O4 was prepared successfully using glassy carbon. The electrochemical measurement is performed for different concentration of glucose. Increment in glucose concentration gives the linear response in form of higher potential.
Graphene Oxide and Copper Ferrite GO/Cu〖Fe〗_2 0_4 Nano-composites as Adsorbent for Fluoride Removal from Aqueous Solution
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2019) SABA RANA; CIIT/FA17-RPH-002/LHR; Dr. Muhammad Hammad Aziz; LHR TP 5781
Water, the universal solvent, is a prerequisite for the existence of all forms of life on the planet earth .60% of the fluid running in the human body is water. Any irregularity in the concentration of minerals can lead to serious health hazards. Such is the case with Fluoride, a major element for human metabolism and its occurrence in drinking water could be safe or unsafe. Fluoride of 0.5 mg L-1 is needed to strengthen tooth polish and moreover to prevent the early tooth decay. However, on exceeding a considerable point, it produces a disease called 'fluorosis'. The present research is centered on the defluoridation of water employing the adsorption method by batch experimental tests. The two-dimensional graphene and graphene-based materials are in effect broadly used being in wastewater treatment as a result of their electronic mobility, incredible surface area, high mechanical strength, great abrasion restraint ability and tunable surface, improved hydrophobicity, adsorption capacity, and recyclability making it ideal material for wastewater treatment. Recently, copper ferrite (CuFe2O4) nanoparticles have drawn various researchers' attention because of its intrinsic properties in application of water purification. CuFe2O4 Nano composites are attractive because of material stability under various conditions. In this study, GO/CuFe2O4 was employed as an adsorbent to dislodge Fluoride (F-1) ions from water by adsorption. The results of three adsorption factors (contact time, pH and Fluoride concentration) on two reaction factors (removal efficiency and adsorption limit) were investigated. The ideal performance conditions for the adsorption of Fluoride (F-1) were noted to be contact time of 75 min, pH of 6 with an initial dose of adsorbent of 10mg/L. The total removal ability and saturation capacity of Fluoride (F-1) under this working condition were observed to be 88% and 2.37mg/g individually. The equilibrium adsorption isotherms and kinetic adsorption models illustrated that the Langmuir isotherm and pseudo-second-order both model showed coherence to the trial information. The characteristics studies of the material were achieved using X-ray diffraction (XRD), Fourier Transform Infrared Spectrometer (FT-IR), Scanning Electron microscope (SEM), Raman, and Ultraviolet-Visible (UV-Vis) spectroscopy.
Green Synthesis of Au doped SnO2 mediated Via Artocarpus Heterophyllus for the Degradation of Methylene Blue Dye in Water Treatment Application
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2022) Hira Khalid; CIIT/FA20-RPH-042/LHR; Dr. Muhammad Hammad Aziz; LHR TP 7998
The green synthesis of nanoscale materials is of special interest to researchers all over the world. We describe a simple, inexpensive, and environment friendly approach to the synthesis of gold-doped tin oxide nanoparticles, which is based on the use of plant extracts. Green synthesized nanoparticles can be used as catalysts, photocatalysts, adsorbents, or alternative agents for the elimination of various organic dyes. With the rapid growth of population, increasing urbanization and industrialization, environmental pollution is becoming a serious concern worldwide. In this work, gold-doped tin oxide nanoparticles were synthesized via sol-gel method. Different type of pollutants is released into the water bodies enormously from the expansive range of industries. Among all the pollutants, dyes are the major used noxious waste discharged by these productions. Dyes are posing a threat to the ecosystem and human health risks.. Green synthesis of nanoparticles (NPs) can be done to degrade molecules of dyes in wastewater. Removal of dyes from the wastewater using green synthesized nanoparticles with the help of plant extracts is a sustainable technique, i.e., by the use of this technique, our environment will not be polluted, and its quality will be maintained. The present review discusses the nanoparticle formation by using plant extract, and, finally, the remediation of dye using such nanoparticles. Overall, the approach presented in the article supports environmental protection and is a promising alternative to other synthesis techniques
Green Synthesis, Characterizations and Photocatalytic Activity of ZnO Nanaoparticles Mediated via Okra Extract
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2020) TANZEEL IQBAL; CIIT/FA17-RPH-005/LHR; Dr. Muhammad Hammad Aziz; LHR TP 6064
The progression of metal oxide nanomaterials through the green union convention has proven to be a leading tool in the application of nanotechnology especially as it is being used for drugs, biosensors, pharmaceuticals and water treatment. Nowadays, green chemical awareness has been observed in cell thinking, biosensors and drug delivery, the manufacture of Nano devices and the elimination of wastewater pollution. The synthesis of nanomaterials in raw form is unstable, unique biocompatibility, low cost and environmentally friendly. The aim of my research project is to find photocatalytic activity test technology with in-depth research into ZnO nanoparticles experiments linked to okra extract. Green synthesis ZnO will dramatically clarify the nanotechnology method using advanced technological instruments, for example, X-beam Diffraction (XRD), Field Emission Scanning Electron Microscope (SEM) and a visible UV spectrometer. This blue-green compound of ZnO nanoparticles will show the degradation of blue dye (MB) before sun exposure.
Preparation of GO-ZnO Nanocomposite and its Antibacterial Activity
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2019) HINA IRUM AMIN; CIIT/FA17-RPH-057/LHR; Dr. Muhammad Hammad Aziz; LHR TP 5780
The progression of nanomaterials demonstrated prevailing apparatuses for nanotechnological sales in various kinds of application like electronics, drug deliver carriers, biosensors and nanomedicine. By and large, nanoparticles appear to be a double-edged sword as they have both valuable and unsafe impacts, depending upon the circumstance of applications. Graphene and its family derivatives like graphene oxide (GO) is the allotrope of carbon and reduced graphene oxide (RGO) also allotrope form of carbon attract in the entire world because of its outstanding physiochemical properties, for example, huge surface area to volume proportion, magnificent charge transportation, great quality and low electrical noise. Graphene based nanomaterial and its composites for example GO-ZnO in a biological point of view require an detailed origination with outstanding antibacterial properties with decline toxicity quality and morphological evaluations of these materials is as yet appealing test for future interest. Here, in this experimental work, we have synthesized and characterized GO-ZnO by X-ray diffraction (XRD), Uv-Vis spectroscopy, Field emission scanning electron microscopy FESEM, for morphological and essential study. Toxicity of GO-ZnO nanocomposite was surveyed by means of methyl-thiazole-tetrazolium (MTT) measure, and morphological changes realize GO-ZnO in Escherichia coli (E.coli) investigated by confocal checking electron microscopy with magnification of ×40. The toxicity of GO-ZnO nanoparticle was assessed by estimating the degree of misfortune in cell viability (%) in dose dependent which caused the cell destruction in apoptotic/necrotic way. The reason of bacterial death due oxidative pressure trigger by reactive oxygen species (ROS) expressed potential role of GO-ZnO for enhancement of antimicrobial activity of E coli as an effective antibacterial specialist
Silver Doped ZnO Nanoparticles for Antimicrobial Activity and Wound Healing Application
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2020) YASIR IQBAL; CIIT/FA18-RPH-031/LHR; Dr. Muhammad Hammad Aziz; LHR TP 6090
Metallic nanoparticles are well known for their biomedical applications including antimicrobial activity and wound dressing materials due to large surface to volume ratio and other chemical properties. In this work, silver doped zinc oxide (Ag-ZnO) nanoparticles containing chitosan-based porous membranes were prepared by the freeze- gelation method. Hydrogel membranes were prepared using the blending of natural polymers and loading metallic nanoparticles as an antibacterial agent. The different membranes were prepared using the different concentrations of metallic nanoparticles by the freeze-gelation process, during this process Triethyl Orthoformate (TEOF) used as a cross-linker agent. This different concentration of membranes were tested for bactericidal potential using the disk diffusion method. Further, swelling studies of these membranes were performed in PBS solutions. The presence of Ag-ZnO NPs were confirmed by the scanning electron microscopy (SEM) analysis. The Fourier Transform Infrared Spectroscopy (FTIR) analysis confirmed the presence of the chitosan functional group. All these experiments confirmed that these membranes are safe to use for wound dressing material and other biomedical applications.
Synthesis and Characterization for Optical and Photocatalytic Activity of Silver doped Lanthanum-Zinc Ferrites in Water Purification under Visible Light
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2021) MUBEEN AKRAM; CIIT/ FA19-RPH-032/LHR; Dr. Muhammad Hammad Aziz; LHR TP 7278
Using the hydrothermal technique lanthanum-Zinc ferrites (ZnLaxFe2-xO4) and silver (1% and 3%) doped Lanthanum-Zinc ferrites (Ag ZnLaxFe2-xO4) at X = 0.2 (for exceptional photocatalytic activity at this concentration [93]), nanoparticles are fabricated. Rh B dye according to its toxicity, color, and environmental hazards was used as aqueous pollutant source for photocatalytic experiment results. The synthesized nanoparticles were characterized by various techniques such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), UV-visible spectroscopy, Scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDAX), Energy dispersive spectroscopy (EDS), and Photoluminescence (PL).The photocatalytic activity of synthesized nanoparticles was studied by conducting the degradation of Rhodamine B (Rh B) under the visible light. PL spectra were verified the recombination and separation time for the synthesized nanoparticles in different light regions. 88.57% of Rh B was degraded in 120 min using silver (3%) doped Lanthanum-Zinc Ferrite. 82.14 % was degraded in 120 min using silver(1%) doped lanthanum-Zinc-ferrites and 71.42% for lanthanum-Zinc ferrite under visible light using 10 mg of the catalyst in 10ppm Rh B solution. The result attests to low cost, reusable and high efficiency photocatalyst for environmental remediation. The synthesize sample is easily separable using any external magnetic source. UV-DRS results and PL spectra revealed exactly the same band gap energy for LZF, ALZF1, and ALZF2 as 2.06, 1.783, and 1.770 eV respectively. Also sample UV results show the shifting of absorption spectrum towards the visible region of light. FTIR and XRD result show the functional and cubic spinel shape photocatalysis formation. SEM images show the morphology of the sample. Reusability and stability experiment exhibit the miner reduction in catalyst efficiency percentage after five cycles. According to extraordinary applications the synthesize sample are use on large scale for IWW treatment process in future
Synthesis of Ag DopedNiO/ZrO2 Core Shell Nanoparticles and Photocatalytic Degradation of Methylene Blue Under UV-Visible Irradiations
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2021) Faiz Muhammad; CIIT/SP20-RPH-042/LHR; Dr. Muhammad Hammad Aziz; LHR TP 7693
A good approach for the photo degradation of Methylene Blue (MB) dye under UV- Visible irradiation is the use of Ag doped nickel oxide –zirconium oxide core shell nanoparticles as a photocatalyst that showed the high photocatalytic activity, highly magnetic separability and cost-effective results. The nanoparticles of Ag doped nickel oxide-zirconium oxide (Ag, NiO/ZrO2, x=1%, 3%) were prepared by using hydrothermal synthesis route. The structural and morphological properties of synthesized nanoparticles studied by X-ray diffraction (XRD), scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), Energy dispersive spectroscopy (EDX), UV- visible spectroscopy, and Photoluminescence (PL). The structural morphology showed that prepared samples are uniform.The structural properties confirmed to be in good agreement for both XRD, and SEM results and confirmed the cubical nature of the prepared spinel. Furthermore, the increase in size of the nanoparticles with increase in Sliver (Ag) contents, confirmed from the diffraction peaks and SEM analysis. The size of crystallites ranged in 29.13nm evaluated by using Debye-Sharer‘s formula from more intense diffraction peak. The compositionof the prepared specimen confirmed by Fourier transform infrared spectroscopy (FTIR).Photocatalysis carried out to check the photocatalytic activity of the synthesized nanoparticles having different concentrations of , for the degradation of Methylene Blue (MB) dye under UV-Visible light. The photocatalytic activity checked, resulted 8%, 27%, 41% and 80% for Sliver doped nickel- zirconium oxide respectively, for the degradation of Methylene Blue (MB) dye under UV-Visible irradiation in 0 ,30 ,60, 90 and 120mins, it is concluded that the photocatalytic activity increased by increasing reaction time and the concentration of Sliver , and this can be increased up to 80 %. The prepared core shell nanoparticles attested to be cost effective, ecofriendly, reusable after recycling, high magnetic x separability after use, and high photocatalytic activity for the photodegradation of Methylene Blue (MB) dye under UV-Visible irradiation.
Synthesis of CeO2/NiFe2O4 Nanocomposites and their Photocatalytic Activity of Methyl Orange Dye under UV-Visible Irradiations
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2022) ALI BASHEER; CIIT/ FA20-RPH-048/LHR; Dr. Muhammad Hammad Aziz; LHR TP 8003
Using the hydrothermal technique CeO2/NiFe2O4 for exceptional photocatalytic activity, nanoparticles are fabricated. Methyl Orange dye according to its toxicity, color, and environmental hazards was used as aqueous pollutant source for photocatalytic experiment results. The synthesized nanoparticles were characterized by various techniques such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), UV-visible spectroscopy, scanning electron microscopy (SEM). The photocatalytic activity of synthesized nanoparticles was studied by conducting the degradation of methyl orange under the visible light. PL spectra were verified for the recombination and separation time for the synthesized nanoparticles in different light regions. 88.57% of Methyl Orange was degraded in 120 min. The result attests to low cost, reusable and high efficiency photo catalyst for environmental remediation. The synthesize sample is easily separable using any external magnetic source. In addition, sample results show the shifting of absorption spectrum towards the visible region of light. FTIR and XRD results show the functional and cubic spinel shape photo catalysis formation. SEM images show the morphology of the sample. Reusability and stability experiment exhibit the miner reduction in catalyst efficiency percentage after five cycles. According to extraordinary applications the synthesize sample are used on large scale for Industrial Waste Water treatment process in future.
Synthesis of Gd Doped Ni-Ferrites and their Photocatalytic Degradation of Methylene Blue Under UV-Visible Irradiations
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2021) MUHAMMAD DILSHAD; CIIT/ FA19-RPH-038/LHR; Dr. Muhammad Hammad Aziz; LHR TP 7265
A good approach for the photodegradation of Methylene Blue (MB) dye under UV-Visible irradiation is the use of Gadolinium-doped Nickel-Ferrites as a photocatalyst that showed the high photocatalytic activity, highly magnetic separability and cost-effective results. The nanoparticles of Gadolinium-doped Nickel-Ferrites (NiGdxFe2-xO4, x=1 %, 3 %, 5%) were prepared by using hydrothermal synthesis route. The structural and morphological properties of the synthesized nanoparticles studied by X-ray diffraction (XRD), scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), Energy dispersive spectroscopy (EDX), UV-visible spectroscopy, and Photoluminescence (PL). The structural morphology showed that prepared samples are uniform. The structural properties confirmed to be in good agreement for both XRD, and SEM results and confirmed the cubical nature of the prepared spinel. Furthermore, the increase in size of the nanoparticles with increase in Gd3+ contents, confirmed from the diffraction peaks and SEM analysis. The size of crystallites ranged in 42-48nm evaluated by using Debye-Sharrer’s formula from more intense diffraction peak. The composition of the prepared specimen confirmed by Fourier transform infrared spectroscopy (FTIR), that showed to sharp absorption bands from 400 cm-1 and 600 cm-1.Photocatalysis carried out to check the photocatalytic activity of the synthesized nanoparticles having different concentrations of Gd3+, for the degradation of Methylene Blue (MB) dye under UV-Visible light. The photocatalytic activity checked, resulted 8%, 27%, 41% and 80% for host nickel ferrites and Gd doped nickel ferrites respectively, for the degradation of Methylene Blue (MB) dye under UV-Visible irradiation only in 50 mins, it is concluded that the photocatalytic activity increased by increasing reaction time and the concentration of Gd3+ in nickel ferrites, and this can be increased up to 80 %. The prepared nanoparticles (nickel ferrites with various Gd3+ contents) attested to be cost effective, eco-friendly, reusable after recycling, high magnetic separability after use, and high photocatalytic activity for the photodegradation of Methylene Blue (MB) dye under UV-Visible irradiation.
Synthesis, Characterization and Antibacterial Properties of Keratin-Bioactive Glass Alveolar Scaffolds
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2020) MUHAMMAD FARRUKH SARFRAZ; CIIT/FA18-RPH-026/LHR; Dr. Muhammad Hammad Aziz; LHR TP 6087
The study was conducted to evaluate the prepared Keratin-Bioactive Glass biomaterial for its potential to act as s scaffold for alveolar grafting. Alveolar bone loss is a common phenomenon associated with periodontal degeneration in tooth extraction processes. The bacterial presence in the oral cavity can result in the failure of the process as the implant is prone to bacterial colonization. Different composite biomaterials have been designed to compensate for the alveolar bone loss. In this study, using the process of freeze gelation method, biocompatible keratin biopolymers and bioactive glass was prepared for its use in alveolar grafting process. Keratin required for the process was extracted from the sheep’s wool, which was blended with bioactive glass composite. The synthesized composite scaffold was then characterized for its structural analysis using FTIR technique. Following the characterization, the scaffold was tested for its mechanical potential and antibacterial activities. The results obtained showed a promising potential for the prepared scaffold to be used as implant alveolar grafting.
Synthesis, Characterization and Antibacterial Study of Silver Doped Manganese Ferrite Nanocomposites
(Library Information Services, COMSATS University Islamabad, Lahore Campus, 2020) BABAR HUSSAIN; CIIT/FA18-RPH-039/LHR; Dr. Muhammad Hammad Aziz; LHR TP 6094
Magnetic materials such as ferrites are substances that come crossways in powder form and ceramic based material with ferromagnetic properties attained via iron oxides (IO), their foremost components are Fe2O3 and Fe3O4 and further can be doped by transition metals and metal oxide nanomaterials . The ferrites and metallic doped ferrites are mentioned as magnetic materials and have various applications such as photocatalysis, drug carrier in cancer treatment, hydrogen production and electronic expedients. Among the various ferrites, manganese ferrite (MnFe2O4) has superior and distinct magnetic, physical and chemical properties leading to numerous biomedical applications. The statistics showed that antibacterial toxicity mostly is influenced by particle size and concentration. The magnetic materials were shown enough potential in antimicrobial activity against various microorganisms and based on these properties that materials can further be used in numerous biomedical applications. Therefore, in present research work, efforts will be carried out by making the nanocomposite of silver doped manganese ferrite NPs to enhancement in the bactericidal action. Silver based nanomaterial and its composites for example Ag- MnFe2O4 in a biological point of view require an detailed origination with outstanding antibacterial properties with decline toxicity quality and morphological evaluations of these materials is as yet appealing test for future interest. Here, in this experimental work, we have synthesized and characterized Ag-MnFe2O4 by X-ray diffraction (XRD), Uv-Vis spectroscopy, Field emission scanning electron microscopy FESEM, for morphological and essential study. Toxicity of Ag-MnFe2O4 nanocomposite was surveyed by means of methyl-thiazole-tetrazolium (MTT) measure, and morphological changes realize Ag-MnFe2O4 in Escherichia coli (E.coli) investigated by confocal checking electron microscopy with magnification of ×40. The toxicity of Ag-MnFe2O4 nanoparticle was assessed by estimating the degree of misfortune in cell viability (%) in dose dependent which caused the cell destruction in apoptotic/necrotic way. The reason of bacterial death due oxidative pressure trigger by reactive oxygen species (ROS) expressed potential role of Ag-MnFe2O4 for enhancement of antimicrobial activity of E coli as an effective antibacterial specialist.