Browsing by Author "Dr. Faiza Sharif"

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Bilayer Electrospun Membrane Modification with Phytochemicals for Wound Healing / Scar Removal
(Library Information Services COMSATS University Islamabad Lahore Campus, 2021-02-26) Ayman Zehra; SP20-R06-005; Dr. Faiza Sharif; LHR TP 7560
The main aim of this work was to synthesize bilayer polymeric membranes through electrospinning and then modify the surface of those membranes with phytochemicals by dip coating. These modified membranes are prospective candidates for wound healing and scar removal. Electrospinning is relatively a new technique to produce nano and micro fibrous membranes having nano and microporous morphology for various biomedical applications. Phytochemicals such as Licorice and Bearberry extracts have been used for centuries in Ayurveda and traditional medicine throughout the world, including Persia, China, India and the Pakistan. These extracts have been found effective in the treatment of wounds, hyperpigmentation, melanin reduction, skin aging, freckles and scars for hundreds of years. Many phytochemicals such as alkaloids, flavonoids, tannins, terpenes, arbutin, glyrrhizic acid, volatile oils and organic acids have been found in the chemical composition of the licorice and bearberry extracts. These compounds possess antibacterial, antioxidant, anti-inflammatory, antimicrobial, and in anticancer activities
Biosynthesized Bacterial Cellulose (BC) Composites; Chemical and Mechanical Characterization
(2025-04-01) Ayesha Nazir; CIIT/FA23-R06-010/LHR; Dr. Faiza Sharif; LHR TP 9686
A new method for crosslinking for Bacterial Cellulose (BC) and Chitosan (CS) hydrogels using Triethyl orthoformate (TEOF) has been developed, resulting in improved mechanical strength cytocompatibility, biocompatibility, biodegradability, and structural integrity. Comprehensive characterizations using FTIR, FESEM, DMA and WCA confirmed the formation and stability of the proposed crosslinked network. The hydrogel, with a 40/60% BC/CS resulted in more porous, sponge-like morphology, have potential biomedical applications, particularly in wound dressings and skin tissue engineering.
Effect of Gamma Irradiation on Mechanical Properties of Bacterial Cellulose Sheets for Wet Wound Dressings
(Library Information Services COMSATS University Islamabad Lahore Campus, 2023-02-27) Aleena Asif; SP22-R06-025; Dr. Faiza Sharif; LHR TP 8663
With advances in material science, new approaches for synthesis and modification utilizing modern techniques have become achievable. Bacterial cellulose (BC), being non-toxic, highly pure, and biocompatible is a versatile biomaterial with various biomedical applications for drug delivery, tissue implants, corneal treatment, and wound dressings. Although BC is capable of improved cell viability and attachment but in its native form it does not show antimicrobial activity. Therefore, it is essential to maintain the sterility of BC based wound dressings by various techniques. Gamma irradiation is a safe sterilization technique. As it has high penetration ability, it is important to analyze its effects on mechanical properties of BC after treatment. Our main interest is the effect of gamma dosages on the integrity of the BC pellicles after exposure. The present work also is on research outcomes of BC in biomedical applications with focus on modern BC based wet dressings for burns and wound healing. In our work, BC was produced by commonly used bacterium Komagataeibacter hansenii. After its production, BC membranes were sterilized by different doses (5KGy,10KGy and 15KGy) of gamma irradiation. The degradation assay was performed in phosphate buffer saline solution for the period of six months. Characterization and comparison of membrane before and after irradiation and degradation in different intervals were performed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscope (SEM) and Dynamic mechanical analysis (DMA), Sterility test, swelling ratio, degradation, antibacterial, drug release assay and cell viability assay.
Synthesis of Polymeric Nanoparticles from Phytochemical Extracts for Activation of Osteogenic Regenerative Effects
(Library Information Services COMSATS University Islamabad Lahore Campus, 2021-02-23) Muneer Ahmad; FA19-R06-008; Dr. Faiza Sharif; LHR TP 7534
The main aim of this work was to synthesize polymeric nanoparticles from chitosan a natural polymer to encapsulate the pomegranate extract via ionic gelation method. Phytochemicals from plants such as Pomegranate (Punica granatum) has been utilized in traditional medicine throughout the world, including Persia, China, and the Indo-Pakistan subcontinent. Pomegranate has been found effective in the treatment of hypertension, diabetes, hyperlipidemia, atherosclerosis, peptic ulcer, oral diseases and several types of cancer for hundreds of years. Many phytochemicals such as alkaloids, flavonoids, tannins, volatile oils and organic acids have been recognized from various parts of the PG, these compounds possess many types of activities like antibacterial, antioxidant, anti-oncogenic, antimicrobial, and in cerebrovascular disease. The PG-NPs were prepared using chitosan shell to encapsulate PG extract in four different ratios i.e. 1:0, 1:0.25, 1:0.75 and 1:1. A study was conducted to evaluate the encapsulation efficiency of the synthesized NPs, and in the same way in vitro drug release and degradation of the NPs was assessed. Characterization was done via UV-VIS spectrophotometry, FTIR, SEM analysis, in vitro biocompatibility test. In vitro biocompatibility was evaluated using Fibroblast cell line NH3T3 by co-culturing cells and NPs for 24 hrs. It was found that PG-NPs were formed with high encapsulation efficiency of PG extract for samples 1:0.25, 1:0.75 and 1:1 were 92%, 57% and 49% respectively. UV-Vis analysis showed the formation of nanoparticles, as well as in vitro drug release within first 5 hours of incubation at 37°C in PBS. The particles were highly biocompatible and even increased the cell proliferation within 24 h. The absorbance analysis as well as visual morphology of the cells co cultured with loaded polymeric NPs suggest that the NPs positively affect the growth of cells in vitro. These PG-NPs possess a better encapsulation efficiency for sensitive degradable drugs. In this way the drugs can be carried to the targeted regions of the body to treat certain disease and to regenerate wounded tissues.