Abstract:
A unique molecularly imprinted polymer (MIP) film was built on a pencil graphite
electrode (PGE), by electropolymerization of pyrrole (monomer) consisting of
acetylcholine (template) and ZnO.CuO nanoleaves (NLs). The sensor was modified for
electrochemical non-enzymatic detection of acetylcholine. Acetylcholine (ACh) serves as
an important neurotransmitter and deviation from its normal level contributes to several
neurological diseases. The MIP’s experimental parameters were optimized, and the
imprinting effect was verified by contrasting the electrochemical reaction of molecularly
imprinted polymer (MIP), with that of non-imprinted polymer (NIP). Among the
electrochemical sensors the MIP (PPy. ZnO.CuO NLs)/PGE electrochemical sensor
manifested the best performance. It showed a wide linear range from 100.0 picomolar (pM)
to 100.0 millimolar (mM). The limit of detection (LOD) and limit of quantification (LOQ)
were shown to be 9.4 pM and 28.68 mM, correspondingly.
Graphical representation of electrochemical sensor for the detection of Acetylcholine
