Abstract:
The periosteum is a dense, well-vascularized membrane that covers the exterior surfaces of
bones, except for the joints. The periosteum is responsible for collagen arrangement and cell
regulation, and it is important for bone integrity, modelling, remodeling particularly in bone
healing. Periosteum transplantation has been used in clinical medicine due to its superior ability
to deliver osteoprogenitor cells while also protecting scar tissues. Immunological rejection,
periosteum availability, and donor side morbidity are, as with previous transplants, the key
issues. It is envisaged that the use of tissue engineering ideas in periosteum-related therapy
would increase the use of periosteum in bone therapies. It can also act as a substitute for natural
periosteum. In this study, we have prepared scaffold for bilayer periosteum incorporated with
Chitosan(CS), Polycaprolactone(PCL), Bioactive glass (BG) and Vitamin E (Vit E) through
Freeze drying and Electrospinning methods. FTIR was used for detection of functional groups
of scaffold. The physiological behavior was also investigated such as degradation, swelling
behavior and porosity. With a suitable degradation rate, the porosity of composite scaffolds
was determined to be in the range 100 percent. Invitro cell studies indicated that scaffold
support growth and cell adhesion. The innovative composite scaffolds will be promising
candidates for bilayer periosteum regeneration.
