Abstract:
Diabetes Mellitus is an aggregation of metabolic disorder in which increase in
blood glucose level is reported for a prolonged period which cause many
complications in humans. Diabetic foot ulcer (DFU) is one of the main
complications which caused by Diabetes Mellitus. Diabetic foot ulcer has severe
medical, economic and social effects for prolonged time. Proper management of
burn wounds is complicated and involves a lot of necessary measures. This study
aims at evaluation of clinical and microbial characteristics of diabetic foot ulcers and
effects of Protease enzyme in curing and healing process of these ulcers. Diabetic
foot ulcers healing is complicated phenomenon which involves many complicated
processes for tissue repairing. The infections caused by the certain bacteria have
become a major challenge in the treatment of foot ulcers and has become associated
with the morbidity and mortality rates in hospitals. Despite the advances in the
medication processes, there is no reliable and successful treatment method for the
diabetic foot ulcers. The most common individual isolates in foot ulcers are
Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Citrobacter
sp. and Escherichia coli. Out of these strains Staphylococcus aureus, Pseudomonas
aeruginosa and Escherichia coli were selected for this study. These organisms were
sensitive as well as were resistant to most of antibiotics. The biofilms of
Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were made
on glass substrata from the samples taken from foot ulcers by using qualitative and
quantitative methods. Activity of crude protease enzyme against the isolates in the
form of biofilm was estimated. This study aims at evaluation of Anti-biofilm activity
of protease enzyme that could be developed as an alternative, fast and safe treatment
method for the diabetic foot ulcers.
