Shaista AhmedFA19-R06-005Dr. Usman LatifLHR TP 73272026-02-252021-02-25https://repository.cuilahore.edu.pk/handle/123456789/2184Cancer is one of the leading cause of death worldwide and delay in diagnosis is one of the major reason of high mortality rate. Among different cancer biomarkers interleukin-8 (IL-8) is considered as useful biomarker for the purpose of quick and accurate diagnosis. A sensitive and selective interdigital transducer based sensor have been fabricated by using molecular imprinted polymer (MIP) technique for the detection of biomarker IL-8. Detection sites synthesized by using functional monomer Methylmethacrylate (MMA), Graphene Oxide GO, cross linker Ethylene glycol dimethacrylate (EGDMA) and IRGACURE blend as a photo initiator. The synthesized sensor showed a high sensitive response for a linear range of IL-8 concentration 1ng/ml to 500ng/ml and coefficient of determination of R2 = 0.954 confirm the linear detection behavior with 239 pg/ml limit of detection. It also showed high selectivity Ribonuclease, IL-6, Lysozyme, Chymotrypsin and Glycoprotein used as control proteins. This sensor showed a unique strategy for detection of IL-8 in buffer as well as in saliva. Scanning electron microscopy (SEM) has been used to analyze the morphology of the fabricated sensor surfaceenDepartment of ChemistryChemistryFA19Dr. Usman LatifCancerbiomarkersMethylmethacrylatedimethacrylateThesis