Synthesis and Characterization of Dipyromethanes and Their Metal Complexes for Glucose Detection

Abstract

Glucose plays an active part in human body such as balancing the body in nonfortified feature, generating energy and improve memory enlargement. Blood glucose concentration is a universal benchmark of a patient’s fitness, particularly for threatening mark related with diabetes mellitus. Due to increased numeral of diabetic patients, various methodologies for glucose determination have been employed to assist precise and uninterrupted glucose detection. In present work, an advanced enzyme free electrochemical device has been proposed for glucose measurements. We intended to synthesize various derivatives of Dipyromethanes (DPM) via microwave assisted synthesis for the detection of Glucose. The synthesis of DPM is generally acid catalyzed reactions that occur in the existence of organic solvent dichloromethane (CH2Cl2). However, the problem of refinement and separation associated with conventional synthetic routs limits their synthesis and utilization in numerous fields. To avoid the lengthy and time consuming process of crystallization and separation we aim to further synthesize their transition metal complexes with Zn. The complexes of DPMs were purified via simple centrifugation. These DPM complexes were further drop casted onto carbon based electrodes (preferably glassy carbon electrodes) to study their electrochemical properties. For this purpose, the electrochemical biosensor was prepared by the modification of a freshly glassy carbon electrode surface with zinc-dipyromethanes complex. The spectral studies were done by UV-visible spectrophotometer and fluorescence spectrophotometer. The surface morphology and structure of the prepared complex were analyzed by AFM, FTIR, and XRD. The electrochemical characteristics of the complex were evaluated by CV and EIS. The results of CV (oxidation peak at 0.33V) indicated that zinc-dipyromethanes complex (Zn-DPM) can efficiently oxidize the glucose without any enzymes assistance and can further be used for glucose detection.